The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S-transferase alpha-4, During the reaction, Glutathione S-transferase alpha-4 in the sample or standard competes with a fixed amount of biotin-labeled Glutathione S-transferase alpha-4 for sites on a pre-coated Monoclonal antibody specific to Glutathione S-transferase alpha-4. Excess conjugate and unbound sample or stan