Fab-pHast mouse is one of our fastest tools for quantitative testing of your primary antibody's specificity, binding, and internalization, providing results in 1 day. Fab-pHast mouse binds to your primary mouse antibody via a secondary antibody cross-linked to a pH-dependent fluorescent reporter. This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, as evident when exposed to the environment inside a cell. A successful assay will provide an EC 50 by way of a fluorescence detecting plate reader, illuminating your lead antibody candidates. Specificity and Preparation: This secondary conjugate recognizes YOUR mouse monoclonal antibody. Fab-pHast is a chemical conjugate of goat anti-mouse monovalent antibody and a pH-dependent fluorescent reporter. The antibody fragments used to make Fab-pHast mouse are affinity-purified polyclonal antibodies against both the heavy and light chain of mouse IgG. The antibody fragment used in this product will cross-react across immunoglobulin classes and subclasses of the same species as they share the same light chain (either kappa or lambda). The pHast fluorescent dye has an excitation wavelength of 532 nm with an emission maxima at 560 nm.
Size
100 ug
Concentration
n/a
Applications
Fab-pHast mouse generates quantitative testing of the specific, binding, and internalization of your primary mouse antibody, with results in 1-day. This secondary conjugate is used to evaluate the potential of a primary antibody to internalize.