Diabetic Mouse Cardiac Fibroblasts from Creative Bioarray are isolated from the heart of Diabetic (db/db) mice (8 weeks). Diabetic Mouse Cardiac Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6cells per ml.