Western Blotting (WB),ELISA,Immunofluorescence (IF)
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Description
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Immunogen
Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from baker's yeast. Freund's complete adjuvant is used in the first step of the immunization procedure.