The CD54 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for CD54 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD54 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD54 Antigens and a biotinylated monoclonal antibody specific for CD54 are simultaneously incubated. Sample Types: serum, plasma, cell culture supernatant. Range: 0.25-8 ng/ml. Sensitivity: 0.1 ng/ml. Time: 1h40 ? The Eagle Biosciences Human sICAM-1/CD54 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule -1 (sICAM-1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human sICAM-1/CD54. The Eagle Biosciences Human sICAM-1/CD54 ELISA Assay kit is for Research Use Only.