eNOS KO Mouse Spleen Endothelial Cells from Creative Bioarray are isolated from tissue of pathogen-free laboratory mice. eNOS KO Mouse Spleen Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Creative Bioarray' Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of Dynabeads pre-coated with secondary antibody.