ELISA, IF Microscopy, Western Blot. This affinity purified antibody has been tested for use in ELISA and western blot. A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.
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Description
This affinity purified antibody is directed against human Bmi1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Bmi1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human Bmi1 protein.