ELISA, IF Microscopy, Immunoprecipitation, Western Blot. This antibody has been tested for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to mouse p19Arf by western blotting in the appropriate cell line or lysate.
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Description
This is a Protein G purified antibody directed against mouse p19Arf protein. No reactivity is observed against human or hamster homologues. The epitope was putatively mapped to amino acids 62-75 of mouse p19Arf. BLAST analysis indicates that no significant sequence homology exists for this sequence with p19Arf homologues from other sources. The homologue from rat only shows 11 of 14 identities (78% homology). No additional information is available for reactivity with p19Arf protein from other sources.
Immunogen
This protein G purified monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 54-75 of mouse p19Arf protein.