Eagle Biosciences hARTN (Human Artemin) test is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with hARTN-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and hARTN molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated hARTN-specific monoclonal detection antibodies bind to the same epitope on another molecule of dimeric hARTN. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the colour produced is directly proportional to the amount of hARTN in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm. Sample Types: serum, plasma, cell culture supernatant. Range: 30 - 1920 pg/mL. Sensitivity: 2 pg/ml. Time: 2 hours ? The Eagle Biosciences Human Artemin ELISA Assay Kit is intended for the quantitative determination of hARTN in serum, plasma, and cell culture supernatant. The Human Artemin ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. The kit has been developed based on unique Artemin antibodies, which are raised against full-length recombinant protein expressed in mammalian cell culture. As is the case with many antibodies, they have high affinity and specificity (no cross reactivity to other neural growth factors).