Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications. It is intended to be determinated 1,25-DVD3 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat 1,25-DVD3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add 1,25-DVD3 and 1,25-DVD3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enz