Rat IgG Specific Fluorescein-Peroxidase Steptavidin-Biotin Complex Immunohistochemistry Detection Kit is a streptavidin-biotin immuno-enzymatic antigen detection system. This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, fluorescein-peroxidase-conjugated streptavidin, and chromogen substrate. Rat Specific FITC-HRP (F-ABC) kit includes reagents specifically designed for the use in the analysis of antigens in IHC and other immunodetection assays. The provided protocol includes steps for high sesitivity analysis with low backgound using normal rabbit serum blocking reagent, biotinylated secondary antibody, and fluorescein-peroxidase-conjugated streptavadin.
Size
1 kit
Concentration
n/a
Applications
Immunohistochemistry. This kit contains concentrated products and will need dilution. Useful for Immunohistochemistry. IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.