Human IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Human IgG Fab fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti- Human IgG and anti- Human IgG F(ab')2. No reaction was observed against anti- Human IgG F(c) or anti-Papain.
Size
2 mg
Concentration
2.0 mg/mL
Applications
Human IgG Fab Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Other Names
Human Immunoglobulin Fab, F(ab), Fragment antigen-binding