The Ansh Labs' IGF-II ELISA kit is a quantitative two-step sandwich immunoassay. Direct detection method eliminates the potential altering of sample matrix from size-exclusion chromatography or filtration methods. The IGF-II assay is calibrated to the WHO IGF-II preparation NIBSC Code 96/538 v4.0. Wide dynamic range of 20 - 1239 ng/mL. Sensitive to 1.33 ng/mL. Total 1.5 hr incubation at room temperature. Long shelf life of 24 months. Research Use Only within the United States. Ansh Labs is an FDA registered manufacturer of diagnostic test kits, antibodies, antigens, and proteins.