This kit was based on Competitive-ELISA detection method. The microtiter plate provided in thiskit has been pre-coated with 8-iso-PGF2? . During the reaction, 8-iso-PGF2? in the sample orstandard competes with a fixed amount of 8-iso-PGF2? on the solid phase supporter for siteson the Biotinylated Detection Antibody specific to 8-iso-PGF2? . Excess conjugate and unboundsample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to eachmicroplate well and incubated. The