Purity: > 95% by SDS - PAGE Format: In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 50mM NaCl, 10% glycerol . NEDD8 belongs to the ubiquitin family and human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The NEDD8 system is essential for the regulation of protein degradation pathways involved in cell cycle progression, morphogenesis and tumorigenesis. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Recombinant human NEDD8 protein was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Size
0.5mg, 100ug, 20ug
Concentration
n/a
Applications
SDS-PAGE
Other Names
Neural precursor cell expressed developmentally down-regulated 8, ubiquitin-like protein Nedd8, Neddylin, Neural precursor cell expressed, developmentally down-regulated 8 MGC104393, MGC125896, MGC125897, NED8, 0NEDD 8, Neddylin, Neural precursor cell expressed developmentally down regulated 8, Neural precursor cell expressed developmentally down regulated gene 8, Rub1, ubiquitin like protein Nedd 8, ubiquitin like protein Nedd8.