Eagle Biosciences human MANF Assay kit is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with human MANF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human MANF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human MANF-specific polyclonal detection antibodies bind to different epitopes of human MANF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the colour produced is directly proportional to the amount of human MANF in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm. Sample Types: serum, plasma, cell culture supernatant. Range: 30 - 75 pg/mL. Sensitivity: 0.25 pg/ml. Time: 2.5 hours ? The Eagle Biosciences MANF ELISA Assay Kit is intended for the quantitative determination of human MANF in serum, plasma, and cell culture supernatant. The MANF ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.