Amplite? Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*. Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent. Peroxynitrite is formed from the reaction between superoxide radicals and nitric oxide generated in cells. It can damage a wide array of biomolecules including proteins, enzymes, lipids and nucleic acids, eventually contributing to cell death. Due to its extremely short half-life and low steady-state concentration, it has been challenging to detect and quantify peroxynitrite in solution. In order to address this need, AAT Bioquest's Amplite? Fluorimetric Peroxynitrite Quantification Kit provides a sensitive tool to measure ONOO- in solution. DAX-J2? PON Green 99 reacts with ONOO- to generate a bright green fluorescent product. It specifically reacts with ONOO- with high selectivity over other reactive oxygen species (ROS) and reactive nitrogen species (RNS). This kit can be used with a fluorescence microplate reader and spectrometer.