ELISA, Immunohistochemistry, Western Blot. GFP Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
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Description
Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Other Names
goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
Tobias Goldmann, Peter Wieghofer, Marta Joana Costa Jordao, Fabiola Prutek, ... Marco Prinz. (2016) Origin, fate and dynamics of macrophages at central nervous system interfaces. Nature Immunology vol 17, pgs 797-805. doi:10.1038/ni.3423.Jain A, Liu R, Xiang YK, Ha T. (2012) Single-molecule pull-down for studying protein interactions. Nat Protoc. 2012 Feb 9;7(3):445-52. doi: 10.1038/nprot.2011.452.