If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY.
Description
Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues . The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development . Studies found that cancer cells exclusively express PKM2 . PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) . When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells . These cells also show decreased tumorigenicity in mouse xenografts . Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 . A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice . These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth . Synonyms: PKM2 , TCB, PK3,PKM2 pyruvate kinase, muscle, CTHBP, PK2