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Description
The rat CD45 antigen (Leukocyte Common Antigen (L-CA)) has been shown to exist in different forms on different lymphoid cell types. CD45 on thymocytes shows one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa. ABIN133310detects a subfraction of the 240 kDa rat CD45 band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX 1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are non-competitive in binding to L-CA (MRC OX 1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX 22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX 22 and OX 31 compete for binding but are non- competitive with OX 32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes. This clone works in flow cytometry and with frozen and paraffin sections (2).