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Description
Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+CD45- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1+ CD45- cells were injected into neonatal liver of busulfan-treated mice. Anti-Mouse PCLP1 stains LO cells as well as the endothelial-like cells in the AGM culture. LO cell is a novel OSM (oncostatin M)-dependent cell line which exhibits characteristics similar to endothelial-like cells. Today it is reported that the PCLP1 is identical with the Podocalyxin.Synonyms: GCTM-2 antigen, Hematopoietic Stem Cell Marker, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1