Flow Cytometry, Immunohistochemistry, Immunoprecipitation. Anti-CD45.1 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.
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Description
Phycoerythrin conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.1. Cross reactivity is observed with human and Feline. Cross-reactivity with CD45.1 from other sources has not been tested. Anti-CD45.1 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Immunogen
Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.
Willinger T and Flavell, RA. 2012. Proc. Natl. Acad. Sci. 109: 8670 - 8675.Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. Wakim LM, Woodward-Davis A, and Bevan MJ. 2010. 107: 17872-17879.