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Description
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin β4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa protein corresponding to the molecular mass of Integrin β4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.
Immunogen
Phospho-Integrin β4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.