Tumorigenecity:Yes, in immunotolerant rats Isoenzyme:Me-2,1;PGM3,1;PGM1,2;ES-D,1;AK-1,1;GLO-1,2;G6PD,B Histopathology:adenocarcinoma Subculture:Remove medium, add with fresh 0.25% trypsin solution for 1 min, remove trypsin and let the culture sit at room temperature for 5 to 10 mins. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days Media change:twice per week Reverse transcritase:Production