Sample Type: Serum, plasma, culture cells, culture solution, tissue and other samples. Intended Use: This kit can measure NO content of all kinds of serum, plasma, culture cells, culture solution, tissue and other samples. Test Principle: NO, O2 and H2O gather together will generate nitrate and nitrite which meet nitrate color developing agent will generate red azoic compound. According to colorimetric test, concentration of NO will be determined. Kit Components (see Protocol for final kit quantities): Reagent 1 Blank tube, 0.8 mLxtube Standard tube, 0.8 mLxtube Sample tube, 0.8 mLxtube Reagent 2 Blank tube, 0.4 mLxtube Standard tube, 0.4 mLxtube Sample tube, 0.4 mLxtube