Primary Human Fibroblast Cells are pooled primary isolates from 250 individual neonatal donor foreskins. These cells were originated using Complete Serum-Free Medium Kit With SuperFuel?, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask. These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks. Each vial or flask of cells is shipped to Customer with Bac-O (antibiotic) and SuperEnergy? (animal derived growth factors) or SuperEnergy-R? (human recombinant growth factors) at no additional cost.