human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other
Isotype
n/a
Format
immunoaffinity purified
Size
400 μg
Concentration
2 mg/ml
Applications
WB, IF, IHC-P, IHC-F
Reviews / Ratings
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Description
Goat polyclonal to GAPDH (glyceraldehyde 3-phosphate dehydrogenase). GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
Immunogen
Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.
1. Sacchi V, Wang BJ, Kubli D, et al. J Am Heart Assoc. 2017 Oct (Supplemental Information). PMID:29018025, 2. Khalafalla FG, Kayani W, Kassab A, et al. J Physiol. 2017 Oct. PMID:28980705, 3. Firouzi F, MSc Thesis, San Diego State University 2017, 4. Goncalves A, Lin CM, Muthusamy A, et al. Invest Ophthalmol Vis Sci. 2016 May. PMID: 27163772, 5. Hyenne V, Apaydin A, Rodriguez D, et al. J Cell Biol. 2015 Oct. PMID: 26459596, 6. Helsby MA, Leader PM, Fenn JR. BMC Cell Biol. 2014 Feb. PMID: 24528853, 7. Grilo MLJ, MSc Thesis, University of Coimbra 2014, 8. Matos CTSM, PhD Thesis, NOVA University of Lisbon 2013