Purity ~90% (SDS-PAGE). G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Source: Recombinant protein corresponding to E.coli mug, fused to His-tag at N-terminal expressed in E.coli. Molecular Weight: ~21.1kD (191aa) (MALDI-TOF) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R Enzym
Size
50ug
Concentration
n/a
Applications
n/a
Other Names
Mug, Recombinant, aa1-168, His-Tag (G/U Mismatch-specific DNA Glycosylase, Xanthine DNA glycosylase, Dug, ECK3058, JW3040, YgjF)