Sample Type: Serum, plasma and other biological fluids. Intended Use: Competitive ELISA, Coated with Antigen Sensitivity: <0.234ng/ml Range: 0.319-25ng/ml Specificity: Human Test Principle: This BioAssay? ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 5-Me-THF. During the reaction, 5-Me-THF in the sample or standard competes with a fixed amount of 5-Me-THF on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 5-Me-THF. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of 5-Me-THF in the samples is then determined by comparing the O.D. of the sampl