Immunofluorescence: 1:50-1:200, Immunohistochemistry (Paraffin): 1:50-1:200, Western Blot: 1:500-1:1000
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Description
Arfaptin 1 Polyclonal Antibody. Arfaptin 1 is an ARF binding protein that inhibits PLD activation, vesicular trafficking and secretion. In the present report, we show that arfaptin 1 interacts with 'high speed' membranes independently of ARF. However, addition of myristoylated ARF3 (myrARF3) increases the association of arfaptin 1 with the membranes, suggesting that arfaptin 1 and ARF form a complex on the Golgi. Utilizing several deletion mutants of arfaptin 1 it is shown that the association of arfaptin 1 with myrARF3 is mediated via two binding sites on arfaptin 1. These two domains are needed for arfaptin 1 inhibition of PLD activation by myrARF3 in vitro.
Immunogen
n/a
Other Names
Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1, Arfaptin1