MW: ~81 kDa. Recombinant full-length human NDR was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: NDR or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family that is regulated by Mob proteins (1). Fas and TNFalpha receptor stimulation activates human NDR by promoting phosphorylation at the hydrophobic motif. NDR acts as a novel pro-apoptotic kinase and key member of the RASSF1A/MST1 sign
1. Millward, T. et.al: Molecular cloning and characterization of a conserved nuclear serine(threonine) protein kinase. Proc. Nat. Acad. Sci. 92: 5022-5026, 1995. 2. Vichalkovski A. et al: NDR kinase is activated by RASSF1A/MST1 in response to Fas receptor stimulation and promotes apoptosis. Curr Biol. 2008 Dec 9;18(23):1889-95.