Primary Human Glomerular Mesangial Cells were initiated by elutriation of dispase dissociated decapsulated glomeruli isolated from normal human cortical kidney tissue. These cells were originated using Complete Serum-Free Medium Kit With SuperFuel?, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5 X 10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.