RecoveryELISA Omalizumab IgE ELISA Assay Kit is performed in a 96-well microplate. The wells of the microplate are pre-coated with a specific capture antibody against human IgE that binds free IgE from the patient sample. There occurs a simultaneous incubation of the IgE levels (with and without the addition of Omalizumab) of the samples and of the detection conjugate (peroxidase conjugate of an anti-IgE antibody). Incubation takes place over 16 to 22 h at 2 to 8°C. After washing, the colour substrate TMB (tetramethylbenzidine) is added to the wells. After incubation the enzymatic colour reaction is stopped using a sulphuric acid solution. A colour change from blue to yellow occurs that is optically detected. Using a wavelength of 450 nm (reference value 620 nm), the optical density (OD) of the reaction product is measured with a suitable microplate reader. Sample Types: serum. Range: Omalizumab: 0.4 - 80 μg/mL; IgE in sample without Omalizumab: 1-2000IU/ml;IgE in sample with Omalizumab: 1-500IU/ml. Sensitivity: Omalizumab: 0.4 μg/mL; IgE: 1 IU/ml. Time: overnight ? The Eagle Biosciences RecoveryELISA Omalizumab IgE ELISA Assay Kit is a test kit for the quantitative determination of free IgE and available Omalizumab under the influence of the available therapeutic antibody Omalizumab in human serum samples. It consists of a manual, non-automated kit for the determination of 7 samples. The Eagle Biosciences Omalizumab IgE ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.