Mouse FGF23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for FGF23 has been precoated onto 96-well plates. Standards(E Coli, Y25-V251) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS