Non-glycosylated protein, containing 175 amino acids, with a molecular weight of 19.5 kDa. Purity is typically 95% as determined by reducing and non-reducing SDS-PAGE. Receptor activator of nuclear factor kappa-B Ligand (RANKL) is a cell-bound marker related to the tumor necrosis factor (TNF) family of proteins. RANKL plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANKL promotes dendritic cell maturation.