This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to GH has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated GH and incubated. A competitive inhibition reaction is launched between GH (Standards or samples) and Biotin-conjugated GH with the pre-coated antibody specific for GH. The more amount of GH in samples, the less antibody bound by Biotin-conjugated GH. Then Avidi