|This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with haptoglobin. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for haptoglobin. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of haptoglobin in samples. The color development is stopped and the intensi