|This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human alpha-CTx. During the reaction,Human alpha-CTx in the sample or standard competes with a fixed amount of Human alpha-CTx on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human alpha-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incub