This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRYAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRYAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRYAA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme
[alpha-crystallin A chain; Alpha-crystallin A chain; alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1); crystallin, alpha A; Heat shock protein beta-4; HspB4]