This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ALCAMantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ALCAMantibody was used as detection antibodies. The standards, test samples and biotin conjugateddetection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidinwas added and unbound conjugates were washed away with wash buffer. TMBsubstrates were used to visualize HRP enzymatic reaction. TMB w