This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with 2-ME2. During the reaction, 2-ME2 in the sample or standard competes with a fixed amount of 2-ME2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 2-ME2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution i