Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Optimal dilution of this antibody should be experimentally determined.
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Description
The RTN1-A/NSP Antibody (MON160) [Alexa Fluor? 350] from Novus Biologicals is a mouse monoclonal antibody to RTN1-A/NSP. This antibody reacts with human, mouse, rat, hamster. The RTN1-A/NSP Antibody (MON160) [Alexa Fluor? 350] has been validated for the following applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Immunogen
Clone MON-160 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with a partially purified bacterially expressed Reticulon 1A (NSP-A) hybrid protein (beta -GAL-NSP-A 6-776). MON-160 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-160 is located between amino acid residues 174-337 of Reticulon-1A.