Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. By Western blot, these antibodies recognize a band 120 kDa, representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120 kDa representing post-translational modification of HIF-1 alpha. Nuclear extracts should be used for Western analysis.
Reviews / Ratings
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Description
The HIF-1 alpha Antibody Pack from Novus Biologicals is a set of mouse antibodies to HIF-1 alpha. These antibodies react with human, mouse, rat, primate. The HIF-1 alpha Antibody Pack has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. These antibodies are specific for HIF-1 alpha
Immunogen
NB100-105, NB100-134 = Fusion protein containing amino acids 432-528 of human HIF-1 alpha. NB100-296 = Fusion protein containing amino acids 329-530 of human HIF-1 alpha. NB100-449 = A synthetic peptide mapping to a region between residues 775 and the C
Other Names
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcription factor)