Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. Western Blot: All antibodies included in this pack will work for Western analysis, except for NB100-131, which has mixed results for Western analysis. A starting dilution of 1:1,000 is recommended with titration up or down depending upon the signal and background. Immunohistochemistry: Both positive and negative feedback have been provided on all antibodies included in this pack. A starting dilution of 1:250 is recommended with titration up or down depending upon the signal and background.
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Description
The HIF-1 alpha Antibody Pack from Novus Biologicals is a set of antibodies to HIF-1 alpha. These antibodies react with human, mouse. The HIF-1 alpha Antibody Pack has been validated for the following applications: Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen. These antibodies are specific for HIF-1alpha
Immunogen
NB 100-654 = Human HIF-1 alpha amino acids 432-528. NB 100-105 = Human HIF-1 alpha amino acids 432-528. NB 100-131 = Human HIF-1 alpha amino acids 329-530. NB 100-449 = Human HIF-1 alpha amino acids 775-826. NB 100-479 = Mouse HIF-1 alpha amino acids
Other Names
ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1 alpha, HIF1A, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1HIF1-ALPHA, PAS domain-containing protein 8, PASD8alpha subunit (basic helix-loop-helix transcription factor)