Titrated value sufficient to run approximately 10 mini blots.
Applications
Western Blot. 14-3-3 antibody is suitable for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to 14-3-3 protein in the appropriate cell lysate or extract.
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Description
Anti-14-3-3 Antibody is directed against rat 14-3-3 protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the peptide that was used to generate the antibody. Reactivity is expected from the following species based on 100% sequence homology: bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish.
Immunogen
Anti-14-3-3 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues from the C-terminal region.
Other Names
14-3-3 protein beta/alpha, Prepronerve growth factor RNH-1, Protein kinase C inhibitor protein 1