Anti PR3 (c-ANCA) ELISA Assay Kit is a test is based on the binding of the antibodies in the sample with human neutrophil proteinase 3 coated into the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind to the inner surface of the wells. After a 60 minutes incubation the microplate is washed with wash buffer for removing non-reactive serum components. In the second step an anti-human-IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized Antigenss. After a 30 minutes incubation any excess enzyme conjugate which is not specifically bound is washed away with the wash buffer. Sample Types: serum, plasma. Range: 10 - 160AU/mL. Sensitivity: 0.13 AU/mL. Time: 1.5 hours ? Anti-neutrophilic-cytoplasm antibodies (ANCA) represents a group of autoantibodies directed towards the cytoplasmatic components of the neutrophilic granulocytes and monocytes. The classical methods for the determination of ANCA are the immunofluorescent methods. With these indirect immunofluorescence techniques two main patterns are recognized, a cytoplasmatic (c-ANCA) and a perinuclear (p-ANCA) type.