The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Rat HAV-IgG in samples. Add sample to wells pre - coated with one Rat Hepatitis A Virus IgG antibody, at same time add HRP-conjugated Rat Hepatitis A Virus IgG antibody to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and colo