tPA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for tPA. Standards or samples are then added to the microtiter plate wells and tPA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of tPA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for tPA are added to each we