Mouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 8 x10^6 cells and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended. Mouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.