The Ansh Labs' Intact IGFBP-4 ELISA is a quantitative three-step sandwich immunoassay. The ratio of Total to Intact IGFBP-4 concentration measured in individual subjects will help normalizes the variability between subjects and also increase the detection rate of increased PAPP-A activity in myocardial infarction subjects. Useful in research related to cancer studies, hypertension, diabetes, cardiomyopathy, osteoarthritis, aging and longevity studies, and growth hormone deficiency. The monoclonal antibody pair used detects intact IGFBP-4. The capture antibody binds to the N terminal of the molecule whereas detection antibody binds to C terminal. The assay does not cross-react to IGF-I, rat IGF-I, IGFBP-3, IGFBP-3/IGF-1 complex and IGFBP-2 at 1000 ng/ml concentration. The assay also detects bovine and equine. Analytical range of 7 - 1200 ng/mL. Simple procedure with no sample preparation. Total 2 hr incubation at RT. Sensitive to 1.62 ng/mL. Research Use Only within the United States.